AbstractMapping and assessment of ecosystem services (ES) is a complex activity which includes spatial data acquisition, its organization into databases and generation of maps for the areas of ES supply and demand. GIS is an integral part of these activities and plays key role for the mapping and assessment of ES. There is a significant advance in the development of various tool and models for mapping and assessment during the last decade. The use of GIS in ecosystem services mapping can take three general approaches, analysis tools built into GIS software packages, disciplinary biophysical models applied for ecosystem service assessment and modeling tools designed specifically for ecosystem service assessment. This paper presents the main advantages of the GIS application in these three approaches through analysis of the available tools, models and techniques. The applications are illustrated by examples of mapping works in different areas in Bulgaria.